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Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay
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Lenti ORF clone of Human serpin peptidase inhibitor clade E nexin plasminogen activator inhibitor type 1 member 1 SERPINE1 transcript variant 2 mGFP tagged
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SERPINF2 GFP tagged Human serpin peptidase inhibitor clade F alpha 2 antiplasmin pigment epithelium derived factor member 2 SERPINF2 transcript variant 3
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Transient overexpression of SERPINE1 NM 001165413 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 25 slides per pack
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Produced in rabbits immunized with purified recombinant Human SerpinB9 rh SERPINB9 Catalog 12838 H07B P50453 Glu2 Pro376 Total IgG was purified by Protein A affinity chromatography
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Standard format: Plasmid sent in bacteria as agar stab
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Standard format: Plasmid sent in bacteria as agar stab
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Produced in rabbits immunized with purified, recombinant Human SerpinB6 (rh SerpinB6; Catalog#12837-H07B; AAB30320.1; Asp 2-Pro 376). SerpinB6 specific IgG was purified by Human SerpinB6 affinity chromatography.
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